Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G.
نویسندگان
چکیده
APOBEC3G catalyzes deamination of cytosines in HIV-1 genome, and restricts the HIV-1 infection. Here, we propose a picomole-scale assay for the detection of DNA deamination catalyzed by APOBEC3G. Our results show the suitability of the developed method for a time course analysis of enzyme-catalyzed DNA modifications.
منابع مشابه
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ورودعنوان ژورنال:
- Chemical communications
دوره 48 99 شماره
صفحات -
تاریخ انتشار 2012